Gydle offers a broad range of services to the genomics research community in health, agriculture, forestry and environmental sciences. Services include experimental design, DNA/RNA sequencing, mapping, assembly, annotation and analysis of genomes and pan-genomes, functional genomics and the analysis of expression, variant and phylogenetic analyses. We also provide data submission to public databases and assistance in writing publications. Our worldwide customer base includes universities, government agencies and private businesses. To request services, write us at services@gydle.com

We provide complete services from the design of the sequencing strategy to the assembly of complete high-quality genomes of micro-organisms, organelles, fungi, animals and plants from the simplest to the most complex. Our genome assembly solutions cover the complete continuum between de novo and reference-initiated assemblies and between targeted and whole-genome approaches.   Our unique hybrid assembly algorithms enable the simultaneous use of sequencing, single-molecule and mapping data to construct pseudomolecules representing entire chromosomes with cross-validation of all the supporting data. We provide submission of annotated genomes and their underlying sequencing data to the public databases.

You have an existing assembly that you want to improve in a targeted or global manner. We will help you finish your genome by improving scaffold continuity, closing gaps, resolving repeats and obtaining locally finished-quality sequence, using our computational tools and possibly adding to the initial data with a selection of the best technologies to complement the original data. We can deliver several iterations of improved assemblies over the length of a project, with complete version tracking of sequences and annotation, enabling you to get the best of your data in real time.

We can provide sequencing services your RNA samples or treat your already sequenced datasets. We build high-quality Gene Catalogues, constructing for each gene a unique canonical transcript sequence able to represent its alternative splicing isoforms and allelic variants with the most complete coding sequence. When a reference genome or DNA‑Seq exists, we can build a comprehensive gene space catalogue with both DNA and transcript representations, intron/exon boundaries and complete protein annotation of gene families.

Our comprehensive genome annotation services include the identification of protein-coding genes, transfer RNAs and ribosomal RNAs, the identification of repeats and transposable elements, and possibly additional features, when more data is available. Examples of additional annotation are non-coding RNA, small-RNA, genetic variants, comparisons to another genome. We provide submission of annotated genomes and their underlying sequencing data to the public databases.

We can provide sequencing services your RNA samples or treat your already sequenced datasets. Using our thorough quality control of the RNA‑Seq data, we will perform isoform identification, abundance quantification, building a reference gene catalogue if needed. For expression analysis, we can deliver gene/isoform counts and perform the quantitative expression analysis for you, adding more refined analyses such as variant discovery and allelic expression quantification as needed.

Our variant analysis services identify SNPs, indels and structural variants with high accuracy using both DNA‑Seq and RNA‑Seq data. Combined with our uniquely accurate NUCLEAR fragment mapping software, it can perform variant discovery and genotyping simultaneously across thousands of individuals, reconstruct haplotypes for any ploidy model, and classify variants according to their sequence context.

We provide services to sequence and assemble complete, finished-quality and fully-annotated organelle genomes from the total DNA of plants and animals. Covering chloroplasts as well as animal, fungal and plant mitochondria, we provide submission of annotated organelle genomes and their underlying sequencing data to the public databases.